概述
在实际的蛋白分离、分析工作中，广大科研工作者经常会遇到某些疏水性蛋白及聚合物改性蛋白在常规SEC柱上出峰状态与理论不符的情况。针对此种情况，赛分科技在SRT系列色谱柱的基础上，专门推出针对该类样品的Sepax SRT-C SEC系列色谱柱。该键合固定相采用的表面修饰技术，通过在高纯度的硅胶基质上键合均匀的纳米厚度中性亲水涂层而制备得到。由于采用可控的化学修饰技术，确保了填料批间有着可靠的重现性。SEC填料采用化学键合技术，表面亲水涂层覆盖完全，因此不仅具有优异的稳定性，而且对蛋白等生物样品的非特异性吸附作用也非常小。同时，大的孔体积保证了高的载样量及优异的分辨率。
SRT-C体积排阻色谱柱优点
高载量及高分辨率 稳定的批间重现性 高的蛋白回收率，且蛋白不易失活 极低的非特异性吸附 专为疏水性蛋白及改性蛋白（如PEG化抗体） 适用于常规生物样品的分离与制备。技术参数：
| 固定相 | SRT-C SEC-150 | SRT-C SEC-300 | SRT-C SEC- 500 |
| 材料 | Neutral, hydrophilic film bonded silica | Neutral, hydrophilic film bonded silica | Neutral, hydrophilic film bonded silica |
| 颗粒大小 | 5 mm | 5 mm | 5 mm |
| 孔径 (Å) | ~ 150 | ~ 300 | ~ 500 |
| 蛋白质分子量范围 | 500 - 150,000 | 5,000 – 1,250,000 | 15,000 – 5,000,000 |
| 水溶性聚合物分子量范围 | 500-25,000 | 1,000-100,000 | 25,000-500,000 |
| pH 稳定性 | 2 – 8.5 (pH 8.5-9.5 can be tolerated temporarily.) | 2 – 8.5 (pH 8.5-9.5 can be tolerated temporarily.) | 2 – 8.5 (pH 8.5-9.5 can be tolerated temporarily.) |
| 反压(psi for a 7.8x300 mm) | ~ 700 | ~ 700 | ~ 700 |
| 压力 (psi) | ~ 4,500 | ~ 3,500 | ~ 3,000 |
| 盐浓度范围 | 20 mM - 2.0 M | 20 mM - 2.0 M | 20 mM - 2.0 M |
| 使用温度 (oC) | ~ 80 | ~ 80 | ~ 80 |
| 流动相兼容性 | Aqueous and organic | Aqueous and organic | Aqueous and organic |
订购信息Sepax SRT-C SEC-150
| 产品名称 | 粒度 | 孔径 | 规格 | 订货号 |
| SRT-C SEC-150 | 5um | 150 Å | 21.5x600mm | 235150-21560 |
| SRT-C SEC-150 | 5um | 150 Å | 21.5x300mm | 235150-21530 |
| SRT-C SEC-150 | 5um | 150 Å | 7.5x600mm | 235150-7560 |
| SRT-C SEC-150 | 5um | 150 Å | 7.8x300mm | 235150-7830 |
| SRT-C SEC-150 | 5um | 150 Å | 7.8x150mm | 235150-7815 |
| SRT-C SEC-150 | 5um | 150 Å | 7.8x50 (Guard) | 235150-7805 |
| SRT-C SEC-150 | 5um | 150 Å | 4.6x300mm | 235150-4630 |
| SRT-C SEC-150 | 5um | 150 Å | 4.6x150mm | 235150-4615 |
| SRT-C SEC-150 | 5um | 150 Å | 4.6x50 (Guard) | 235150-4605 |
| 产品名称 | 粒度 | 孔径 | 规格 | 订货号 |
| SRT-C SEC-300 | 5um | 300 Å | 21.5x600mm | 235300-21560 |
| SRT-C SEC-300 | 5um | 300 Å | 21.5x300mm | 235300-21530 |
| SRT-C SEC-300 | 5um | 300 Å | 7.5x600mm | 235300-7560 |
| SRT-C SEC-300 | 5um | 300 Å | 7.8x300mm | 235300-7830 |
| SRT-C SEC-300 | 5um | 300 Å | 7.8x150mm | 235300-7815 |
| SRT-C SEC-300 | 5um | 300 Å | 7.8x50 (Guard) | 235300-7805 |
| SRT-C SEC-300 | 5um | 300 Å | 4.6x300mm | 235300-4630 |
| SRT-C SEC-300 | 5um | 300 Å | 4.6x150mm | 235300-4615 |
| SRT-C SEC-300 | 5um | 300 Å | 4.6x50 (Guard) | 235300-4605 |
| 产品名称 | 粒度 | 孔径 | 规格 | 订货号 |
| SRT-C SEC-500 | 5um | 500 Å | 21.5x600mm | 235500-21560 |
| SRT-C SEC-500 | 5um | 500 Å | 21.5x300mm | 235500-21530 |
| SRT-C SEC-500 | 5um | 500 Å | 7.5x600mm | 235500-7560 |
| SRT-C SEC-500 | 5um | 500 Å | 7.8x300mm | 235500-7830 |
| SRT-C SEC-500 | 5um | 500 Å | 7.8x150mm | 235500-7815 |
| SRT-C SEC-500 | 5um | 500 Å | 7.8x50 (Guard) | 235500-7805 |
| SRT-C SEC-500 | 5um | 500 Å | 4.6x300mm | 235500-4630 |
| SRT-C SEC-500 | 5um | 500 Å | 4.6x150mm | 235500-4615 |
| SRT-C SEC-500 | 5um | 500 Å | 4.6x50 (Guard) | 235500-4605 |